Mouse Lin-28 Homolog A ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Lin-28 Homolog A in biofluid samples, such as tissue homogenates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . LIN28A present in samples reacts with an anti-Mouse LIN28A capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-LIN28A detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active LIN28A in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse LIN28A. Detection Target: Mouse Lin-28 Homolog A Reactivity: Mouse Range: 0.156-10ng/mL Sensitivity: 0.049ng/mL Storage Conditions: Store all components at 4┬░C This Mouse LIN28A activity assay is for the quantitative determination of Lin-28 Homolog A in samples including tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.